Functional analysis in the KIF7 gene expression

JRCT ID: jRCT1071230117

Registered date:16/02/2024

Basic Information

• Recruitment status: Pending
• Health condition(s) or Problem(s) studied: PAC syndrome
• Date of first enrollment: 16/02/2024
• Target sample size: 4
• Study type: Interventional

Intervention(s)

I gather 14 mL blood of a patient of the PAC syndrome, and a control, in the study purpose concerned newly.
I measure serum IL-1 beta, IL-6, TNF-alpha density by ELISA.
I isolate PBMC.
1)It is stimulation with TLR ligand(TL4, TLR9, TLR1/2, TLR2, TLR2/6, TLR3, TLR5, TLR7, TLR8).
2) I gather supernatant liquid of PBMC which I stimulated, and PBMC not to stimulate, and measure IL-1 beta, IL-6, TNF-alpha density.
3) I separate RNA from PBMC which I stimulated, and PBMC not to stimulate. I reverse-transcript it to cDNA and, using molds such as KIF7, perform rtPCR.
I make SiRNA of the KIF7-related factor and introduce a gene into PBMC by transfection and check a change including the expression of KIF7.

Outcome(s)

• Primary Outcome: Change before and after the stimulation of mRNA and the CT
• Secondary Outcome: the detection level of the ELISA including TNF-alpha, IL-6, and IL-1 beta, and more

Key inclusion & exclusion criteria

• Age minimum: >= 20age old
• Age maximum: Not applicable
• Gender: Both

Include criteria

object person(patient)
In Kurume University Hospital collagenosis internal medicine, I intend for a patient under treatment for PAC syndrome.
The one where a written agreement was provided for study entry from the person
a person 20 years or older

I choose one to meet all the above-mentioned standards

control
The one where a written agreement was provided for study entry from the person
a person 20 years or older

I choose one to meet all the above-mentioned standards

Exclude criteria

object person(patient)
The one that a manager for study and a study partaker judged to be inappropriate as a study subject
The pregnant woman or pregnancy with the possibility

control
The person who had a diagnosis of a self-inflammatory syndrome
The pregnant woman or pregnancy with the possibility

Related Information

• Primary Sponsor: Hidaka Yukiko