Identification of a single parainfluenza-specific lymphocyte in tonsillar focal disease and verification of its involvement in the pathogenesis with single-cell RNA-sequencing

JRCT ID: jRCT1050220136

Registered date:19/12/2022

Basic Information

• Recruitment status: Recruiting
• Health condition(s) or Problem(s) studied: IgA nephropathy, palmoplantar pustulosis, sleep apnea syndrome, tonsillar hypertrophy
• Date of first enrollment: 19/12/2022
• Target sample size: 50
• Study type: Observational

Outcome(s)

Primary Outcome

(1) Medical record information
Age, sex, name of the disease for which palato- tonsillectomy was indicated, blood test results, and bacterial test results (taken from tonsil crypts and tonsil parenchyma).
(2) Information obtained from the removed tonsil tissue and peripheral blood
1. Bacteriological profile of the tonsil crypts and surface by comprehensive genetic analysis.
2. Quantitative analysis of gene expression by single cell analysis of tonsils and peripheral blood mononuclear cells.
3. Quantitative analysis of gene expression in tonsils and peripheral blood mononuclear cells after CpG-ODN stimulation by single cell analysis.

Key inclusion & exclusion criteria

• Age minimum: Not applicable
• Age maximum: Not applicable
• Gender: Both

Include criteria

(1)Patients who have indicated for palatotonsillectomy for the treatment of IgA nephropathy as tonsillar focal disease.
(2) Patients who have indicated for palatotonsillectomy for the treatment of palmoplantar pustulosis as tonsillar focal disease.
(3) Patients who have indicated for palatotonsillectomy with sleep apnea syndrome or enlarged palatine tonsils to relieve upper airway stenosis.

Exclude criteria

(1) Patients with enlarged tonsils due to neoplastic lesions such as malignant tumors (tonsil cancer, malignant lymphoma, etc.) and benign tumors (papilloma of the pharynx), or suspected patients
(2) Patients undergoing emergency palatotonsillectomy due to severe bacterial infection such as peritonsillar abscess and recurrent tonsillitis
(3) Other patients deemed inappropriate to participate in the study.

Related Information

• Primary Sponsor: Kaneko Fumie