Influence of new carbon dioxide paste on "artificial Bohr effect"

UMIN ID: UMIN000038187

Registered date:05/10/2019

Basic Information

• Recruitment status: Pending
• Health condition(s) or Problem(s) studied: healthy male adult
• Date of first enrollment: 2019/11/01
• Target sample size: 10
• Countries of recruitment: Japan
• Study type: Interventional

Intervention(s)

Attach an air tourniquet generally used in orthopedic surgery to the upper arm and attach the NIRS probe to the center of the forearm flexion side (non-dominant hand) with double-sided tape and surgical tape. Stop hemostasis 2 minutes after starting NIRS measurement The band was expanded to a pressure of 250 mmHg. The tourniquet was depressurized 20 minutes after the expansion, and then the measurement was continued for 5 minutes until it returned to a steady state. The measurement was terminated 27 minutes after the start of NIRS measurement. The duration of expansion was 20 minutes.
After the end of Experiment 1, the patient took a 10-minute rest period to recover to a steady state. After confirming the subject's numbness, pain, and absence of abnormal sensation, Experiment 2 was started. As in Experiment 1, the tourniquet was expanded to a pressure of 250 mmHg 2 minutes after the start of NIRS measurement. Carbon dioxide paste was applied to the forearm 8 minutes after pressurizing the tourniquet. The pressure was reduced and measurement was continued for 5 minutes.

Outcome(s)

• Primary Outcome: Changes in oxyhemoglobin and deoxyhemoglobin in the forearm muscle are measured by near infrared spectroscopy (NIRS)

Key inclusion & exclusion criteria

• Age minimum: 20years-old
• Age maximum: 30years-old
• Gender: Male
• Exclude criteria: Person with heart disease, respiratory disease, peripheral nerve disease, skin disease, blood disease.

Related Information

• Primary Sponsor: kobe university
• Source(s) of Monetary Support: kobe university